Western blot protocol wikipedia

Southern blot

The western blot (sometimes called the protein immunoblot), or western blotting, is a widely used analytical technique in molecular biology and immunogenetics to detect specific proteins in a sample of tissue homogenate or extract.

What is western blotting The Western blot is a scientific process. It is a test. Western blotting is also called immunoblotting. Scientists use Western blots to look at proteins. A Western blot can show how big a protein is. Sometimes a Western blot can show how much of the protein is there. Scientists studying proteins do Western blots more than any other test.

Western blot steps A western blot experiment, or western blotting, is a routine technique for protein analysis. The specificity of the antibody-antigen interaction enables a target protein to be identified in the midst of a complex protein mixture.

Western blot analysis The most common method of transfer in western blotting is electrophoretic transfer, where an electric field is used to elute proteins from gels and transfer them to membranes. During this process, the membrane and gel are placed together, with filter paper between two electrodes.

Western blot wikipedia The southwestern blot, is a lab technique that involves identifying as well as characterizing DNA-binding proteins [1] by their ability to bind to specific oligonucleotide probes. Determination of molecular weight of proteins binding to DNA is also made possible by the technique.

Western blot principle

We know that western blotting protocols vary depending on your particular laboratory set-up and reagents of choice. Here are a series of protocols, each explicit to a specific combination of reagents, detection modes, and laboratory equipment. Please use the filters below to select the specific protocol that best fits your needs.

Western blot interpretation A western blot is a laboratory method used to detect specific protein molecules from among a mixture of proteins. This mixture can include all of the proteins associated with a particular tissue.

Northern blotting

Western blotting, also called protein blotting or immunoblotting, uses antibodies to identify specific protein targets bound to a membrane; the specificity of the antibody-antigen interaction enables a target protein to be identified in the midst of a complex protein mixture, such as cell or tissue lysate.